Journal
/
Bioquímica
/
Zuivering van Biotinylated Cell Surface Proteins uit<em> Rhipicephalus microplus</em> Epitheliale darmcellen
JoVE Revista
Bioquímica
Author Produced
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Please note that all translations are automatically generated. Click here for the English version.

Zuivering van Biotinylated Cell Surface Proteins uit<em> Rhipicephalus microplus</em> Epitheliale darmcellen

9,259 Views

•

12:03 min

•

July 23, 2017

DOI:

10.3791/55747-v

DOI:
10.3791/55747-v

•

12:03 min
July 23, 2017

•

3 Views
, ,
1Queensland Alliance for Agriculture & Food Innovation,The University of Queensland, 2Centre for Comparative Genomics,Murdoch University

Summary

Automatically generated

Een gemodificeerde dichtheidscentrifugatiegradient gebaseerde methodologie werd gebruikt om epitheelcellen van Rhipicephalus microplus gutweefsel te isoleren. Oppervlakte gebonden eiwitten werden biotinyleerd en gezuiverd door streptavidine magnetische kralen voor gebruik in downstream toepassingen.

Explore More Videos

Biotinylated Cell Surface ProteinsRhipicephalus MicroplusEpithelial Gut CellsPercoll GradientCell DisassociationCell PurificationStreptavidin Magnetic Beads

Read Article

Cite this Article

Karbanowicz, T. P., Lew-Tabor, A., Rodriguez Valle, M. Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells. J. Vis. Exp. (125), e55747, doi:10.3791/55747 (2017).

Download .ris file

Copy

Share Video

.

Copy