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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

DOI:
10.3791/54672-v

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08:56 min

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December 05, 2016

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, ,
1Department of Cardiology,Chang Gung Memorial Hospital, Chang Gung University College of Medicine

Capítulos

  • 00:05Título
  • 01:10RNA Isolation, Qualification, and Quantification
  • 02:29Gel Electrophoresis
  • 03:51Transfer
  • 06:03Detection of N6-methyladenosine
  • 06:50Immunoblotting
  • 07:59Results: Clear Detection of RNA Methylation
  • 08:29Conclusion

Summary

Traducción Automática

Traducción Automática

A modified northern blotting method for measuring N6-methyladenosine (m6A) modifications in RNA is described. The current method can detect modifications in diverse RNAs and controls under various experimental designs.

Tags

RNA N6-methyladenosineRNA EpigeneticsNorthern BlottingRNA ModificationsN6A DetectionRNA IsolationAgarose Gel ElectrophoresisMOPS BufferFormaldehydeSSC BufferRNA Transfer

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