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Genetics

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JoVE Journal Genetics
Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
 

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Article DOI: 10.3791/55473-v • 10:19 min • April 8th, 2017
April 8th, 2017 •
1, 1
1Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh

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Galaxy and DAVID have emerged as popular tools that allow investigators without bioinformatics training to analyze and interpret RNA-Seq data. We describe a protocol for C. elegans researchers to perform RNA-Seq experiments, access and process the dataset using Galaxy and obtain meaningful biological information from the gene lists using DAVID.

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Transcriptomic Analysis C. Elegans RNA Sequencing Data Tuxedo Suite Galaxy Project Bioinformatics Expertise Open Access Platform High Throughput Sequencing Data Transcriptomic Signatures Phenotypes Differentially Expressed Genes Gene Ontology Terms Web-based Usage Galaxy Pipeline Local Workflow User Interface Tools Used In Workflow Analyze Data
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