Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
Kapitel
- 00:05Titel
- 00:56Getting Started with Galaxy
- 03:28Data Analysis with Tuxedo Suite
- 06:19Gene Ontology (GO) Term Analyses Using DAVID
- 07:56Results: Transcriptome Dictated by the Transcription Elongation Factor TCER-1, that Extends Lifespan of Germline-less C. elegans
- 09:39Conclusion
Galaxy and DAVID have emerged as popular tools that allow investigators without bioinformatics training to analyze and interpret RNA-Seq data. We describe a protocol for C. elegans researchers to perform RNA-Seq experiments, access and process the dataset using Galaxy and obtain meaningful biological information from the gene lists using DAVID.
