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产妇基因产品的官能化操作使用<em>体外</em斑马鱼>卵母细胞成熟
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
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产妇基因产品的官能化操作使用<em>体外</em斑马鱼>卵母细胞成熟

DOI:
10.3791/55213-v

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10:39 min

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April 22, 2017

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, , , ,
1Laboratory of Genetics,University of Wisconsin-Madison, 2Department of Biological Sciences,Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Capítulos

  • 00:05Título
  • 00:50Pre-selection of Females and Preparation of Maturation Medium
  • 02:29Dissection of Oocytes and Initiation of In Vitro Culture
  • 04:56Oocyte Microinjection
  • 05:52Oocyte Maturation and Defolliculation
  • 07:18Fertilization of In Vitro-cultured Oocytes
  • 08:15Results: Maternal Gene Product Manipulation Using In Vitro Oocyte Maturation
  • 09:36Conclusion

Summary

Traducción Automática

Traducción Automática

对于用于产妇的基因产物的操纵斑马鱼的卵母细胞的体外成熟的优化协议这里提出。

Tags

In Vitro Oocyte MaturationZebrafishMaternal Gene ProductsBiología del desarrolloFertilizationViable EmbryosMating TankLeibovitz’s L-15 MediumDHPGentamicinTricaineOocyte DissectionLight Cycle

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