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Optimization of a Quantitative Micro-neutralization Assay
Capítulos
- 00:05Título
- 00:59Virus Titration
- 04:25Titration Quantitation
- 05:59Virus Neutralization
- 07:20Neutralization Quantification
- 08:04Results: Micro-neutralization Optimization
- 09:34Conclusion
This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.
