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Generering<em> De Novo</em> Antigenspecifikke humane T-celle receptorer ved retroviral transduktion af Centric Hemichain

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Generering<em> De Novo</em> Antigenspecifikke humane T-celle receptorer ved retroviral transduktion af Centric Hemichain

8,312 Views

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08:48 min

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October 25, 2016

DOI:

10.3791/54697-v

DOI:

10.3791/54697-v

•

08:48 min

October 25, 2016

•

5 Views

Tingxi Guo², Toshiki Ochi*², Munehide Nakatsugawa*², Yuki Kagoya, Mark Anczurowski², Chung-Hsi Wang², Muhammed A. Rahman, Kayoko Saso, Marcus O. Butler², Naoto Hirano²

¹Department of Immunology,University of Toronto, ²Princess Margaret Cancer Centre,University Health Network

Tags

De Novo T Cell Receptor TCR Hemi-chain Retroviral Transduction Antigen-specific T Cells Gene Engineering Immunotherapy PCR Cloning Bacterial Transformation Plasmid Purification 293GPG Cell Transfection PG-13 Packaging Cell Line PBMC Activation

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