A Method for Measuring RNA <em>N</em><sup>6</sup>-methyladenosine Modifications in Cells and Tissues

Chao-Yung Wang; Mei-Hsiu Lin; Hui-Ting Su

doi:10.3791/54672

Journal

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生物化学

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A Method for Measuring RNA N⁶-methyladenosine Modifications in Cells and Tissues

JoVE 杂志
生物化学

This content is Free Access.

JoVE 杂志生物化学

A Method for Measuring RNA N⁶-methyladenosine Modifications in Cells and Tissues

10,581 Views

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08:56 min

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December 05, 2016

DOI:

10.3791/54672-v

DOI:

10.3791/54672-v

•

08:56 min

December 05, 2016

•

7 Views

Chao-Yung Wang¹, Mei-Hsiu Lin¹, Hui-Ting Su¹

¹Department of Cardiology,Chang Gung Memorial Hospital, Chang Gung University College of Medicine

Summary

A modified northern blotting method for measuring N⁶-methyladenosine (m⁶A) modifications in RNA is described. The current method can detect modifications in diverse RNAs and controls under various experimental designs.

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RNA N6-methyladenosine RNA Epigenetics Northern Blotting RNA Modifications N6A Detection RNA Isolation Agarose Gel Electrophoresis MOPS Buffer Formaldehyde SSC Buffer RNA Transfer

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Summary

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A Method for Measuring RNA N⁶-methyladenosine Modifications in Cells and Tissues