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Ein Fluoreszenz-basierte Assay von Phospholipid Scramblase Activity

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

A Fluorescence-based Assay of Phospholipid Scramblase Activity

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Ein Fluoreszenz-basierte Assay von Phospholipid Scramblase Activity

13,160 Views

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09:52 min

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September 20, 2016

DOI:

10.3791/54635-v

DOI:

10.3791/54635-v

•

09:52 min

September 20, 2016

•

147 Views

Birgit Ploier¹, Anant K. Menon¹

¹Department of Biochemistry,Weill Cornell Medical College

Summary

We describe a fluorescence-based assay to measure phospholipid scrambling in large unilamellar liposomes reconstituted with opsin.

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