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A Fluorescence-based Assay of Phospholipid Scramblase Activity
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Biochemie
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JoVE Journal Biochemie
A Fluorescence-based Assay of Phospholipid Scramblase Activity

A Fluorescence-based Assay of Phospholipid Scramblase Activity

DOI:
10.3791/54635-v

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09:52 min

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September 20, 2016

•

,
1Department of Biochemistry,Weill Cornell Medical College

Kapitel

  • 00:05Titel
  • 00:45Preparation of Liposomes and Proteoliposomes
  • 02:12Quantification of Phospholipids
  • 03:37Reconstitution of Opsin
  • 06:16Scramblase Activity Assay
  • 07:29Results: Reconstitution of Opsin into LUVs to Characterize Its Scramblase Activity
  • 09:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe a fluorescence-based assay to measure phospholipid scrambling in large unilamellar liposomes reconstituted with opsin.

Tags

Phospholipid Scramblase ActivityFluorescence-based AssayG Protein Coupled ReceptorLipid BilayerPOPCPOPGLarge Unilamellar VesiclesExtrusionPerchloric AcidAmmonium Molybdate

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