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A Fluorescence-based Assay of Phospholipid Scramblase Activity
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
A Fluorescence-based Assay of Phospholipid Scramblase Activity
DOI:

09:52 min

September 20, 2016

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Capítulos

  • 00:05Título
  • 00:45Preparation of Liposomes and Proteoliposomes
  • 02:12Quantification of Phospholipids
  • 03:37Reconstitution of Opsin
  • 06:16Scramblase Activity Assay
  • 07:29Results: Reconstitution of Opsin into LUVs to Characterize Its Scramblase Activity
  • 09:09Conclusion

Summary

Traducción Automática

We describe a fluorescence-based assay to measure phospholipid scrambling in large unilamellar liposomes reconstituted with opsin.

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