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星爆无长突细胞的膜片钳在Deafferentated小鼠视网膜的平板安装准备

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Neurociencias

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JoVE Revista Neurociencias

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

Please note that all translations are automatically generated. Click here for the English version.

星爆无长突细胞的膜片钳在Deafferentated小鼠视网膜的平板安装准备

DOI:

10.3791/54608-v

•

08:44 min

•

October 13, 2016

•

Hung-Ya Tu, Chih-Chun Hsu², Yu-Jiun Chen, Ching-Kang Chen^2,3

¹Department of Ophthalmology,Baylor College of Medicine, ²Department of Neuroscience,Baylor College of Medicine, ³Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Capítulos

00:05Título
01:03Retina Dissection
03:30Whole-cell Patch Clamp Recording from Flat-mount Retina
06:34Results: Morphology and Membrane Potentials of ON-and Off-Starburst Amacrine Cell in the Nob Mouse Retina
07:19Conclusion

Summary

Traducción Automática

此协议演示了如何从一台安装在准备视网膜神经元进行全细胞膜片钳记录。

Tags

Keywords: Patch Clamp Starburst Amacrine Cells Flat-mount Deafferentated Mouse Retina Retinal Neurobiology Retinal Circuits Whole-cell Recording Nitrocellulose Membrane External Solution Carbogenated Upright Microscope

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