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Un protocollo dettagliato per caratterizzare la linea cellulare murina C1498 e il suo modello associato leucemia mouse
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Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
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Un protocollo dettagliato per caratterizzare la linea cellulare murina C1498 e il suo modello associato leucemia mouse

19,133 Views

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08:00 min

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October 14, 2016

DOI:

10.3791/54270-v

DOI:
10.3791/54270-v

•

08:00 min
October 14, 2016

•

36 Views
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1Institut pour la Recherche sur le Cancer de Lille (IRCL),INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille

Summary

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Questo manoscritto fornisce una procedura tecnica che può essere utilizzato per caratterizzare colture cellulari C1498 in vitro e la leucemia acuta indotta nei topi dopo l'iniezione. Fenotipiche e funzionali analisi vengono eseguite utilizzando citometria a flusso, microscopia a immunofluorescenza, citochimica e maggio-Grünwald Giemsa.

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Mopin, A., Driss, V., Brinster, C. A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model. J. Vis. Exp. (116), e54270, doi:10.3791/54270 (2016).

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