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How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
JoVE Revista
Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

9,352 Views

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13:18 min

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September 20, 2016

DOI:

10.3791/54230-v

DOI:
10.3791/54230-v

•

13:18 min
September 20, 2016

•

2 Views
, , , , , , , , ,
1Departamento de Genética, Facultad de Medicina,Universidad de la República (UDELAR), 2Department of Mechanistic Cell Biology,Max Planck Institute of Molecular Physiology, 3School of Biological, Biomedical & Environmental Sciences,University of Hull

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Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.

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Basement MembraneStiffnessThicknessAdvanced GlycationProstate CancerMetastasisAge-related PathologiesMetabolic DiseasesCell Invasion3D Cell CultureCross-linkingBiophysical Trigger

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Rodriguez-Teja, M., Breit, C., Clarke, M., Talar, K., Wang, K., Mohammad, M. A., Pickwell, S., Etchandy, G., Stasiuk, G. J., Sturge, J. How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases. J. Vis. Exp. (115), e54230, doi:10.3791/54230 (2016).

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