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Isolering av Murine Embryonic Hemogenic endotelceller
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June 17, 2016
DOI:
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, 3Department of Molecular and Cellular Biology,Baylor College of Medicine
Summary
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Blodkreft stamceller og stamceller (HSPC) stammer fra spesialiserte (hemogenic) endotelceller under utvikling, men lite er kjent om prosessen der noen endotelceller spesifisere å bli bloddannende. Vi viser en flow-cytometri basert fremgangsmåte som tillater samtidig isolering av hemogenic endotelceller og HSPC fra muse-embryonale vev.
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Cite this Article
Fang, J. S., Gritz, E. C., Marcelo, K. L., Hirschi, K. K. Isolation of Murine Embryonic Hemogenic Endothelial Cells. J. Vis. Exp. (112), e54150, doi:10.3791/54150 (2016).
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