Journal
/
/
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
DOI:

08:27 min

October 01, 2016

, , , ,

Capítulos

  • 00:05Título
  • 00:48Förster Resonance Energy Transfer (FRET) Biosensor Preparation
  • 04:25Analysis of Sugar Content of Beverages Using a FRET Biosensor
  • 06:31Results: Quantitative Analysis of Sugar Content Using a Protein Biosensor for Portable FRET Analyzer
  • 07:49Conclusion

Summary

Traducción Automática

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

Videos relacionados

Read Article