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HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
 

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Article DOI: 10.3791/54124-v • 11:29 min • July 20th, 2016
July 20th, 2016 •
1, 1
1Department of Medical Sciences, Human Genetics Foundation (HuGeF), University of Turin

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The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.

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Keywords: HPLC Nucleotide Metabolism Nucleoside Metabolism Chronic Lymphocytic Leukemia CLL Cells Purine Metabolism Adenosine Extracellular Environment Cell Culture Density Gradient Centrifugation PBMC Magnetic Beads Cell Purification
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