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一步法负色谱纯化<em>幽门螺杆菌</em>中性粒细胞激活蛋白过度表达<em>大肠杆菌</em>在批处理模式
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JoVE Revista Inmunología y contagio
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
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一步法负色谱纯化<em>幽门螺杆菌</em>中性粒细胞激活蛋白过度表达<em>大肠杆菌</em>在批处理模式

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10:44 min

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June 18, 2016

DOI:

10.3791/54043-v

DOI:
10.3791/54043-v

•

10:44 min
June 18, 2016

•

1 Views
, , , ,
1Institute of Molecular and Cellular Biology,National Tsing Hua University, 2Department of Life Science,National Tsing Hua University

Summary

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用于重组幽门螺杆菌一步法负纯化的高产量方法，通过使用被描述在批处理模式二乙氨基树脂幽门在大肠杆菌中过表达嗜中性粒细胞激活蛋白（HP-NAP）。通过该方法纯化的HP-NAP是疫苗，药物或诊断为H的发展有利幽门螺杆菌机相关的疾病。

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Helicobacter PyloriNeutrophil-activating ProteinHP-NAPEscherichia ColiNegative Chromatographic PurificationBatch ModeDEAE Ion Exchange ResinProtein PurificationAggregation PreventionBradford Assay

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Kuo, T., Hong, Z., Tsai, C., Yang, Y., Fu, H. One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode. J. Vis. Exp. (112), e54043, doi:10.3791/54043 (2016).

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