Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.
Amend, S. R., Valkenburg, K. C., Pienta, K. J. Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation. J. Vis. Exp. (110), e53936, doi:10.3791/53936 (2016).