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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
JoVE Revista
Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

18,482 Views

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09:25 min

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October 14, 2016

DOI:

10.3791/53884-v

DOI:
10.3791/53884-v

•

09:25 min
October 14, 2016

•

4 Views
, , , , , , , , ,
1Department of Surgery and Translational Medicine (DCMT),University of Florence, 2Neurofarba Department,University of Florence, 3Department of Traumatology and General Orthopedics,Azienda Ospedaliera Universitaria Careggi

Summary

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The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.

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Palmini, G., Zonefrati, R., Mavilia, C., Aldinucci, A., Luzi, E., Marini, F., Franchi, A., Capanna, R., Tanini, A., Brandi, M. L. Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma. J. Vis. Exp. (116), e53884, doi:10.3791/53884 (2016).

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