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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

JoVE 杂志
癌症研究

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JoVE 杂志癌症研究

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

18,349 Views

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09:25 min

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October 14, 2016

DOI:

10.3791/53884-v

DOI:

10.3791/53884-v

•

09:25 min

October 14, 2016

•

8 Views

Gaia Palmini, Roberto Zonefrati, Carmelo Mavilia, Alessandra Aldinucci, Ettore Luzi, Francesca Marini, Alessandro Franchi, Rodolfo Capanna, Annalisa Tanini, Maria Luisa Brandi

¹Department of Surgery and Translational Medicine (DCMT),University of Florence, ²Neurofarba Department,University of Florence, ³Department of Traumatology and General Orthopedics,Azienda Ospedaliera Universitaria Careggi

Tags

Cancer Stem Cells Osteosarcoma Sarcosphere In Vitro Cell Culture Cell Isolation Cell Suspension Growth Factors EGF BFGF Ultra-low Attachment Plates Hemocytometer Phase Contrast Microscopy

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