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Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
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Capítulos
- 00:05Título
- 00:50Rat Brain Dissection
- 04:20Enzymatic and Mechanical Tissue Dissociation
- 05:26Anti-A2B5 Bead Application, Column Purification, and Plating
- 06:29Induction of OPC Differentiation and Fixation with 4% Paraformaldehyde
- 07:38Immunocytochemistry and Qualification of Myelin Basic Protein
- 09:25Results: OPC Culture and Differentiation Procedure
- 10:29Conclusion
Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.
