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Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

DOI:
10.3791/53764-v

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11:11 min

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February 17, 2016

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, , ,
1Neurology,Johns Hopkins University, School of Medicine

Kapitel

  • 00:05Titel
  • 00:50Rat Brain Dissection
  • 04:20Enzymatic and Mechanical Tissue Dissociation
  • 05:26Anti-A2B5 Bead Application, Column Purification, and Plating
  • 06:29Induction of OPC Differentiation and Fixation with 4% Paraformaldehyde
  • 07:38Immunocytochemistry and Qualification of Myelin Basic Protein
  • 09:25Results: OPC Culture and Differentiation Procedure
  • 10:29Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.

Tags

Oligodendrocyte Progenitor CellsOligodendrogenesisSpinal Cord InjuryNeonatal HypoxiaDemyelinating DiseasesTransverse MyelitisMultiple SclerosisRat PupsDissectionPBSPetri DishCraniumCerebellumOlfactory BulbsHypothalamusMidbrainHippocampus

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