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Kwantificering van draadvormige actine (F-actine) Puncta in Rat corticale neuronen
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JoVE Revista Neurociencias
Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
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Kwantificering van draadvormige actine (F-actine) Puncta in Rat corticale neuronen

11,744 Views

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10:13 min

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February 10, 2016

DOI:

10.3791/53697-v

DOI:
10.3791/53697-v

•

10:13 min
February 10, 2016

•

3 Views
, , , ,
1Laboratory Program in Behavioral, Neuroscience, Department of Psychology,University of South Carolina

Summary

Automatically generated

Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.

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Li, H., Aksenova, M., Bertrand, S. J., Mactutus, C. F., Booze, R. Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons. J. Vis. Exp. (108), e53697, doi:10.3791/53697 (2016).

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