Journal
/
Inmunología y contagio
/
Isolering af Exosomer fra plasma af HIV-1 Positive Personer
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolering af Exosomer fra plasma af HIV-1 Positive Personer

DOI:
10.3791/53495-v

•

06:46 min

•

January 05, 2016

•

, , , , , ,
1Department of Microbiology, Biochemistry, Immunology,Morehouse School of Medicine, 2Department of Medicine,Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine,Emory University School of Medicine, 4Yerkes National Primate Research Center

Capítulos

  • 00:05Título
  • 00:54Human Blood Processing
  • 02:20Purification of Exosomes
  • 03:41Acetylcholinesterase Activity Assay
  • 04:50Results: Characterization of Exosomes from HIV-1 Positive Human Plasma
  • 06:10Conclusion

Summary

Traducción Automática

Traducción Automática

Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.

Tags

ExosomesHIV-1PlasmaVirus ParticlesUltracentrifugationIodixanol GradientVirus-infected CellsMicrovesiclesHIV InfectionCentrifugationPBS WashVirus Separation

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

In vitro Uncoating af HIV-1 kerner

Nye værktøjer til at udvide regulatoriske T celler fra HIV-1-inficerede personer

Konformationel Evaluering af HIV-1 trimere kappeglycoproteinerne hjælp af en celle-ELISA-assay

Nucleocapsid Annealing-medieret Electrophoresis (NAME) Indhold Tillader hurtig identifikation af HIV-1 nucleocapsid inhibitorer

Visualisering af HIV-1 Gag binding til Giant unilamellare vesikel (GUV) Membraner

Read Article