Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
قياس درجة الحموضة يبلوع التي كتبها Ratiometric المجهر مضان
13,784 Views
•
14:39 min
•
December 07, 2015
DOI:
Summary
Automatically generated
Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.
Read Article
Cite this Article
Nunes, P., Guido, D., Demaurex, N. Measuring Phagosome pH by Ratiometric Fluorescence Microscopy. J. Vis. Exp. (106), e53402, doi:10.3791/53402 (2015).
Copy