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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
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JoVE Revista Neurociencias
Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

9,955 Views

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10:31 min

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September 29, 2017

DOI:

10.3791/53369-v

DOI:
10.3791/53369-v

•

10:31 min
September 29, 2017

•

9 Views
, , ,
1University of Michigan Medical School, 2Department of Neurology,University of Michigan, 3Department of Internal Medicine,University of Michigan

Summary

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This protocol uses three-dimensional (3D) imaging and analysis techniques to visualize and quantify nerve-specific mitochondria. The techniques are applicable to other situations where one fluorescent signal is used to isolate a subset of data from another fluorescent signal.

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Cite this Article

Hamid, H. S., Hayes, J. M., Feldman, E. L., Lentz, S. I. Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers. J. Vis. Exp. (127), e53369, doi:10.3791/53369 (2017).

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