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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
Kapitel
- 00:05Titel
- 01:02Fluorescence Immunohistochemistry: Day 1
- 02:27Fluorescence Immunohistochemistry: Day 2
- 03:21Fluorescence Immunohistochemistry: Day 3
- 04:22Confocal Imaging
- 06:00Image Processing
- 07:03Isolation of Nerve-specific Mitochondrial Signal
- 08:41Results: Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
- 09:39Conclusion
This protocol uses three-dimensional (3D) imaging and analysis techniques to visualize and quantify nerve-specific mitochondria. The techniques are applicable to other situations where one fluorescent signal is used to isolate a subset of data from another fluorescent signal.
