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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
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JoVE Revista Biología del desarrollo
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

DOI:
10.3791/53301-v

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10:16 min

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February 14, 2016

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, ,
1Department of Agricultural, Food and Nutritional Science,University of Alberta

Capítulos

  • 00:05Título
  • 01:12Grinding Embryos
  • 02:35Genomic DNA Preparation Using a Modified Sodium Hydroxide Method
  • 04:40Design Sex-specific PCR Primers and Genomic DNA PCR
  • 07:34Results: Porcine Embryo Sex Determination
  • 08:54Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.

Tags

DNA SexingPCRPorcine EmbryosPhenol-freeReproductive PhysiologySex RatioDNA PurificationEmbryo SamplesLiquid NitrogenSodium HydroxideDNA Lysate

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