This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.
Blanes, M. S., Tsoi, S. C., Dyck, M. K. Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR. J. Vis. Exp. (108), e53301, doi:10.3791/53301 (2016).