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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
 

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Article DOI: 10.3791/53252-v • 11:37 min • November 24th, 2015
November 24th, 2015 •
1, 1,2, 1, 1, 1
1Department of Genetics, University of Cambridge, 2Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences

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We have developed a protocol to generate aggregates of mouse embryonic stem cells that display self-organization, symmetry breaking and elongation paralleling axial development. This technique allows the study of axial developmental processes and the generation of cell types that are otherwise difficult to perform in monolayer culture.

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Aggregates Mouse Embryonic Stem Cells Symmetry Breaking Polarization Emergent Collective Behavior In Vitro Self-organization Axial Elongation Cell Fate Specification Suspension Culture Gene Expression Morphology Germ Layers Gastrulation-like Movements Wnt/β-Catenin Activation BMP Inhibition Fluorescent Microscopy Whole-mount Mouse Embryo Staining Immunocytochemical Analysis
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