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Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
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Capítulos
- 00:05Título
- 01:47Antibiotic Minimum Inhibitory Concentration (MIC) Assay in 96-well Plates
- 05:33Spot Plate Assay for Oxidative Stress Response
- 09:11Results: Inhibition of Pyomelanin Production Increases Sensitiity to Oxidative Stress
- 10:32Conclusion
Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).
