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Métodos para inibir bacteriana Pyomelanin Produção e determinar o correspondente aumento de sensibilidade ao estresse oxidativo
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Métodos para inibir bacteriana Pyomelanin Produção e determinar o correspondente aumento de sensibilidade ao estresse oxidativo
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August 31, 2015
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Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).
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Ketelboeter, L. M., Bardy, S. L. Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress. J. Vis. Exp. (102), e53105, doi:10.3791/53105 (2015).
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