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Grensesnitt 3D Engineered Nevronale kulturer til Micro-elektrode Arrays Et nyskapende<em> In Vitro</em> Experimental Model
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
DOI:

09:47 min

October 18, 2015

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Capítulos

  • 00:05Título
  • 02:08Preparation of Materials and Supports
  • 04:18Dissection of Embryos, Tissue Dissociation, and Cell Plating
  • 06:323D Neuronal Network Construction
  • 07:28Results: Examples of 3D Networks Captured by Confocal Microscopy and by Electrophysiological Recordings
  • 08:24Conclusion

Summary

Traducción Automática

In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.

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