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La detección de ARNm axón localizado en las secciones cerebrales Uso de alta resolución<em> In Situ</em> La hibridación
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Capítulos
- 00:05Título
- 01:26Heat-Induced Antigen/RNA Unmasking
- 02:21Probe Hybridization
- 04:01Immunofluorescence Staining
- 05:42Diaminobenzidine (DAB)-based Chromogenic ISH Assay Followed by Luxol Fast Blue and Cresyl Violet Stain Using Heat-induced and Protease-induced RNA Unmasking
- 08:54Results: Aft4 Localization to Cholinergic Axons in the Mouse Dentate Gyrus and Myelinated Axons in the Adult Hippocampal Formation
- 10:53Conclusion
RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.
