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适用于比色金纳米粒子检测方法的选择结构切换适配方法
JoVE Revista
Biología
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JoVE Revista Biología
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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适用于比色金纳米粒子检测方法的选择结构切换适配方法

14,680 Views

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12:31 min

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February 28, 2015

DOI:

10.3791/52545-v

DOI:
10.3791/52545-v

•

12:31 min
February 28, 2015

•

2 Views
, , , , ,
1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory,Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.

Tags

Structure-switching AptamersSmall Molecule TargetsBiosensingGold Nanoparticle AssayNucleic Acid AptamersTarget-induced Conformational ChangeNegative SelectionColorimetric ReadoutPhysiological Fluids

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Martin, J. A., Smith, J. E., Warren, M., Chávez, J. L., Hagen, J. A., Kelley-Loughnane, N. A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay. J. Vis. Exp. (96), e52545, doi:10.3791/52545 (2015).

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