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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
JoVE 杂志
生物学
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JoVE 杂志 生物学
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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12:31 min

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February 28, 2015

DOI:

10.3791/52545-v

DOI:
10.3791/52545-v

•

12:31 min
February 28, 2015

•

5 Views
, , , , ,
1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory,Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.

Summary

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A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.

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Structure-switching AptamersSmall Molecule TargetsBiosensingGold Nanoparticle AssayNucleic Acid AptamersTarget-induced Conformational ChangeNegative SelectionColorimetric ReadoutPhysiological Fluids

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Cite this Article

Martin, J. A., Smith, J. E., Warren, M., Chávez, J. L., Hagen, J. A., Kelley-Loughnane, N. A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay. J. Vis. Exp. (96), e52545, doi:10.3791/52545 (2015).

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