Vorbereitung adhärenter Zellen für die Röntgenfluoreszenzbildgebung durch chemische Fixierung

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.