Journal
/
Biología del desarrollo
/
İnsan Mezenkimal Kök Hücre İzolasyonu ve Gözenekli Kemik Matrix kendi Yetiştirme
JoVE Revista
Biología del desarrollo
This content is Free Access.
JoVE Revista Biología del desarrollo
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Please note that all translations are automatically generated. Click here for the English version.

İnsan Mezenkimal Kök Hücre İzolasyonu ve Gözenekli Kemik Matrix kendi Yetiştirme

27,111 Views

•

09:00 min

•

February 09, 2015

DOI:

10.3791/51999-v

DOI:
10.3791/51999-v

•

09:00 min
February 09, 2015

•

15 Views
, , , , , , , ,
1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales,Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química,Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular,Cinvestav-IPN, 6Subd. de Investigación,Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

Summary

Automatically generated

Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.

Explore More Videos

Mesenchymal Stem CellsAM-hMSCsBone MatrixNukboneScaffoldCell DifferentiationBiomaterialsCell Culture

Read Article

Cite this Article

Rodríguez-Fuentes, N., Reynoso-Ducoing, O., Rodríguez-Hernández, A., Ambrosio-Hernández, J. R., Piña-Barba, M. C., Zepeda-Rodríguez, A., Cerbón-Cervantes, M. A., Tapia-Ramírez, J., Alcantara-Quintana, L. E. Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix. J. Vis. Exp. (96), e51999, doi:10.3791/51999 (2015).

Download .ris file

Copy

Share Video

.

Copy