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골수 세포 역학의 실시간 영상<em> APC<sup> 최소 / +</sup</em> 소장 종양 디스크 공 초점 현미경을 회전시켜
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
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골수 세포 역학의 실시간 영상<em> APC<sup> 최소 / +</sup</em> 소장 종양 디스크 공 초점 현미경을 회전시켜

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05:21 min

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October 06, 2014

DOI:

10.3791/51916-v

DOI:
10.3791/51916-v

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05:21 min
October 06, 2014

•

8 Views
, ,
1Department of Oncology,INSERM U661, Functional Genomic Institute, 2Department of Anatomy,University of California

Summary

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By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.

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Real-time ImagingMyeloid CellsIntestinal TumorsSpinning Disk Confocal MicroscopyApcMin/+ MiceC-fms-EGFPACTB-ECFPIntravital ImagingTumor MicroenvironmentIntestinal AdenomasBlood VesselsNeutrophils

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Cite this Article

Bonnans, C., Lohela, M., Werb, Z. Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy. J. Vis. Exp. (92), e51916, doi:10.3791/51916 (2014).

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