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における骨髄細胞ダイナミクスのリアルタイムイメージング<em>のApc<sup>最小/ +</sup</em>腸腫瘍ディスク共焦点顕微鏡スピニングすることにより
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Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
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における骨髄細胞ダイナミクスのリアルタイムイメージング<em>のApc<sup>最小/ +</sup</em>腸腫瘍ディスク共焦点顕微鏡スピニングすることにより

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05:21 min

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October 06, 2014

DOI:

10.3791/51916-v

DOI:
10.3791/51916-v

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05:21 min
October 06, 2014

•

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1Department of Oncology,INSERM U661, Functional Genomic Institute, 2Department of Anatomy,University of California

Summary

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By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.

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Real-time ImagingMyeloid CellsIntestinal TumorsSpinning Disk Confocal MicroscopyApcMin/+ MiceC-fms-EGFPACTB-ECFPIntravital ImagingTumor MicroenvironmentIntestinal AdenomasBlood VesselsNeutrophils

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Cite this Article

Bonnans, C., Lohela, M., Werb, Z. Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy. J. Vis. Exp. (92), e51916, doi:10.3791/51916 (2014).

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