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باستخدام الحمض النووي الريبي التدخل للتحقيق في الاستجابة المناعية الفطرية في الفأر الضامة
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
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باستخدام الحمض النووي الريبي التدخل للتحقيق في الاستجابة المناعية الفطرية في الفأر الضامة

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12:47 min

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November 03, 2014

DOI:

10.3791/51306-v

DOI:
10.3791/51306-v

•

12:47 min
November 03, 2014

•

15 Views
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1Integrated Department of Immunology and Integrated Center for Genes, Environment, and Health,National Jewish Health and University of Colorado School of Medicine

Summary

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In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.

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RNA InterferenceMacrophageInnate Immune ResponseGene KnockdownSiRNA TransfectionAmaxa Nucleofector96-well FormatLipopolysaccharideCytokine Production

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De Arras, L., Guthrie, B. S., Alper, S. Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages. J. Vis. Exp. (93), e51306, doi:10.3791/51306 (2014).

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