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Non Replikatif Kanin adenovirüs tip 2 türetilmiş vektörler üretimi ve saflaştırılması
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Non Replikatif Kanin adenovirüs tip 2 türetilmiş vektörler üretimi ve saflaştırılması
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14:55 min
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December 03, 2013
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Summary
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These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
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Szelechowski, M., Bergeron, C., Gonzalez-Dunia, D., Klonjkowski, B. Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors. J. Vis. Exp. (82), e50833, doi:10.3791/50833 (2013).
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