Journal
/
Inmunología y contagio
/
Assays for the Identification of Novel Antivirals against Bluetongue Virus
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Assays for the Identification of Novel Antivirals against Bluetongue Virus

Assays for the Identification of Novel Antivirals against Bluetongue Virus

DOI:
10.3791/50820-v

•

12:02 min

•

October 11, 2013

•

, ,
1Division of Infectious Diseases, Department of Medicine,University of Alabama at Birmingham, 2Molette Laboratory for Drug Discovery, Department of Chemistry and Biochemistry,Auburn University

Capítulos

  • 00:05Título
  • 01:37Cytopathic Effect Based Assay Using the CellTiterGlo Kit
  • 03:31Dose Response Assay
  • 06:28Time-of-Addition Assay
  • 08:55Representative Antiviral Compound Assay Results
  • 11:34Conclusion

Summary

Traducción Automática

Traducción Automática

Three assays, including the cytopathic effect (CPE)-based assay, dose-response assay and Time-of-Addition (ToA) assay have been developed, optimized, validated and utilized to identify novel antivirals against Bluetongue virus (BTV), as well as to determine the possible Mechanism-of-Action (MoA) for newly identified antivirals.

Tags

Antiviral AssaysBTV AntiviralsCytopathic Effect (CPE) AssayDose-response AssayMechanism Of Action (ToA) AssayAntiviral ScreeningViral LifecycleCell Culture System

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Read Article