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一个<em在体外</em从成年小鼠的肌间神经丛制备隔离肠道神经元和神经胶质
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Neurociencias
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JoVE Revista Neurociencias
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
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一个<em在体外</em从成年小鼠的肌间神经丛制备隔离肠道神经元和神经胶质

26,095 Views

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10:34 min

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August 07, 2013

DOI:

10.3791/50688-v

DOI:
10.3791/50688-v

•

10:34 min
August 07, 2013

•

18 Views
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1Pharmacology and Toxicology,Virginia Commonwealth University, 2Physiology and Biophyics,Virginia Commonwealth University

Summary

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We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

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Smith, T. H., Ngwainmbi, J., Grider, J. R., Dewey, W. L., Akbarali, H. I. An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse. J. Vis. Exp. (78), e50688, doi:10.3791/50688 (2013).

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