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蛍光を用いたmRNA分子の可視化と分析<em>その場で</emで>ハイブリダイゼーション<em>サッカロミセス·セレビシエ（Saccharomyces cerevisiae）</em
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Biología
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JoVE Revista Biología
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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蛍光を用いたmRNA分子の可視化と分析<em>その場で</emで>ハイブリダイゼーション<em>サッカロミセス·セレビシエ（Saccharomyces cerevisiae）</em

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

•

07:00 min
June 14, 2013

•

3 Views
, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Summary

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このプロトコルは、蛍光を実行するための実験手順を説明<em>その場で</em単一分子の解像度で単一細胞中のmRNAを計数する>ハイブリダイゼーション（FISH）。

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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Cite this Article

McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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