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Visualisation et d'analyse de molécules d'ARNm par fluorescence<em> In Situ</em> Hybridation dans<em> Saccharomyces cerevisiae</em
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Biología
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JoVE Revista Biología
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualisation et d'analyse de molécules d'ARNm par fluorescence<em> In Situ</em> Hybridation dans<em> Saccharomyces cerevisiae</em

34,355 Views

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

•

07:00 min
June 14, 2013

•

3 Views
, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Summary

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Ce protocole décrit une méthode expérimentale pour effectuer Fluorescence<em> In situ</em> Hybridation (FISH) pour compter ARNm dans des cellules individuelles à une résolution de molécule unique.

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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Cite this Article

McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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