/
/
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
10,376 Views
•
13:54 min
•
April 02, 2013
DOI:
Summary
Automatically generated
This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
Read Article
Cite this Article
Drennan, S. L., Lama, A., Doron, B., Cambronne, E. D. Tractable Mammalian Cell Infections with Protozoan-primed Bacteria. J. Vis. Exp. (74), e50300, doi:10.3791/50300 (2013).
Copy