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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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April 02, 2013
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This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
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Drennan, S. L., Lama, A., Doron, B., Cambronne, E. D. Tractable Mammalian Cell Infections with Protozoan-primed Bacteria. J. Vis. Exp. (74), e50300, doi:10.3791/50300 (2013).
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