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Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
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Bioingeniería
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JoVE Revista Bioingeniería
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

26,317 Views

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07:32 min

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June 15, 2012

DOI:

10.3791/4248-v

DOI:
10.3791/4248-v

•

07:32 min
June 15, 2012

•

3 Views
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1Department of Biomedical Engineering,Cornell University, 2BioCytics, Inc., 3Carolina BioOncology Institute, PLLC

Summary

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Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.

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Hughes, A. D., Mattison, J., Powderly, J. D., Greene, B. T., King, M. R. Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples. J. Vis. Exp. (64), e4248, doi:10.3791/4248 (2012).

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